what is the order of a blood draw

Citrate Tube Light Blue SST TM Gel Separator Tube Red or Gold or Serum Tube Plastic or Glass - Red PST TM Gel Separator Tube Green or Heparin Tube Sodium or Lithium - Green EDTA Tube Lavender Fluoride Tube Grey. All tubes must be gently inverted 8 10 times to provide thorough mixing of additives.

What Is The Phlebotomy Order Of Draw Phlebotomy Info

Blood samples are collected using collection tubes and this had to be done in a specific order.

. Correct order of draw. Order of blood draw. One yel-low for pediatrics TB Fungal collect yellow isolator tube PT APTT Coagula-tion Studies green NOTE.

Blood Draw Order Tube Color Description. When collecting blood using a syringe and a transfer device is used. These blood tubes may contain different additives which may affect certain results should they contaminate another tube.

Blood Cultures Sterile Tubes. Blood Gas Syringe EDTA tubes. Third non-additive tube red top Last draw additive tubes in this order.

Blue If using a butterfly draw a blue waste tube Black top o ESR CBC etc. Phlebotomy order of draw is the same for specimens collected by syringe tube holder or into tubes preevacuated at the time of collection. Draw one green and one orange bottle for adults.

Contamination of serum samples with K EDTA will occur routinely if order of draw is not followed. Shaking the tube vigorously may cause hemolysis of the blood separating of red blood cells. During syringe collections contamination with K EDTA may rarely occur if order of draw is not followed.

Order of Draw When Using Evacuated Tubes. The additive inside the tube stops the blood from clotting. Blood Culture Bottles Used for bacterial and fungal cultures.

The phlebotomy order of draw is a specific order blood samples must be drawn in based on the additives in the blood collection tubes. 1 Blood cultures yellow SPS sterile 2 Light blue buffered sodium citrate tube 3. In the era of lyophilized anticoagulants order of draw is no longer important.

The draw order for specimen tubes is as. When collecting blood via a capillary collection method heelfinger prick or when using a syringe and siphoning the bloods directly into the tubes the Order of Draw in order is. So in order for blood to clot in safer plastic tubes manufacturers coat the inside of the tube with a substance to facilitate clotting like silica particles.

Cross contamination depending on the tests ordered could cause seriously erroneous results improper patient treatment and possibly death. The recommended order of draw is as follows. When collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on Clinical and Laboratory Standards Institutes CLSI standard H3-A6 from November 2007 as follows.

Sed Rate Westegren Sed Rate sion Fill tube to line. For this reason an order of draw was established. Blood Collection ORDER OF DRAW MIXING CHART SEPARATION Clot Spin Pour into Transport Vial Label with specimen type and patient ID For other blood serum or plasma separation instructions see the Specimen Collection and Handling section of this directory.

What happens if you draw blood in the wrong order. The recommended order of draw for plastic collection tubes is. Heparin either sodium or lithium E thylene D iamine T etra a cetic Acid AKA EDTA Potassium Oxalate with Sodium Fluoride.

Necessitating the change was the industry-wide transition from glass blood collection tubes to plastic. It is based on the Clinical Laboratory Standards Institute CLSI venipuncture standard. The above order of draw is to be followed.

Order of draw is essential in avoiding cross contamination from additives of one tube to the next. Must be drawn first to. Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures.

Whereas glass is a natural clot activator plastic is not. First blood culture bottle or tube yellow or yellow-black top Second coagulation tube light blue top. However the initial evidence that contamination was a problem was based on a study from 1982 using.

What is the order of blood collection tubes. When a patient requires blood tests often more than one tube is required. Order of Draw for Vacutainer Collection Tubes 1.

This tube is collected when testing for coagulation studies such as Von Willebrand disease most common blood clotting disorder Clotting factors D-dimer to rule out thrombosis Anti factor Xa to monitor patients on high dose low molecular weight heparin Prothrombin time etc. Sodium Polyanethol Sulfanate AKA SPS Sodium Citrate. Blood culture tube or bottle.

This order of draw has changed over the years the last occurring in 2003. Phlebotomy Order of Draw. The reason for this precise procedure is to avoid cross-contamination of the various additives between tubes.

The very first thing that each phlebotomist has to pay attention to is to perform blood collection in a specific way. The correct order of draw follows. Blood Draw Order Tube Color NameAdittive of Inversions 1 Varies Blood Cultures 8 10 2 Light Blue Sodium Citrate 3 4 3 Red Clot Activator 4 5 4 Gold SST Serum Separator Tube 4 5 5 Light Green Lithium Heparin 8 10 6 Dark Green.

CLSI order of draw is as follows.

Pin Page